Immunohistochemical evaluation of psoriasis and other proliferative disorders of the skin.

by John Patrick Harford

Publisher: The Author] in [S.l

Written in English
Published: Pages: 94 Downloads: 995
Share This

Edition Notes

Thesis (M. Sc. (Biomedical Sciences)) - University of Ulster, 1993.

The Physical Object
Pagination[vi], 94p., 20p. of plates, tables :
Number of Pages94
ID Numbers
Open LibraryOL21162569M

Our objective was to compare by quantitative immunohistochemistry the subsets of T-lymphocytes and markers for epidermal growth and keratinisation in biopsies taken from skin lesions of 4 patients with psoriasis and 3 patients with ILVEN: 1. patients with psoriasis (case ) 2. patient with ILVEN cum psoriasis (case 5) 3. patients with ILVEN.   INTRODUCTION. Psoriasis is one of the most frequent skin disorders, affecting about % of the general population [].Psoriasis represents a life-long patients’ affliction, with a considerable impact on the quality of life [2, 3].Psoriasis is characterized by epidermal hyperproliferation, abnormal terminal keratinocyte differentiation and inflammation [].   Psoriasis is a chronic inflammatory skin disorder that is characterised by disfiguring, scaling and erythematous plaques that may be itchy or painful. Although once thought of as a benign dermatological condition with few serious complications, psoriasis is now considered a multisystem inflammatory disease that is associated with, or increases. Immunohistochemical and histochemical stains are useful adjunct techniques in the diagnosis of canine cutaneous round cell tumors, which can appear histologically similar We applied a panel of monoclonal antibodies (recognizing tryptase, chymase, serotonin for mast cells; CD1a, CD18, MHC class II for histiocytes; CD3 for T lymphocytes; CD79a for B lymphocytes and plasma cells) and one.

Abnormal histone modification by histone deacetylases (HDACs), including HDAC1 and sirtuin 1 (SIRT1), has been reported to play an important role in the pathogenesis of psoriasis by altering cell proliferation, differentiation, and inflammation. However, findings on the expression level of HDACs in psoriatic skin lack consistency. We assessed the expression of HDAC1, SIRT1, p63, and. (Reed-Sternberg-like cells, asterisks) among the dense cellular infiltrate (H&E X). Immunohistochemical evaluation revealed, among other markers, positivity for CD2 c) and CD4 d), (immunohistochemical stain X). findings, a diagnosis of MTX-related non-specific ulce-rative stomatitis was rendered, possibly induced by the.   Psoriasis, a chronic papulosquamous inflammatory skin disease, was originally thought of as a primary disorder of epidermal keratinocytes, but is later on recognized as one of the commonest immune-mediated disorders [1, 2].Although psoriasis starts with the involvement of skin, but limiting this disease to a skin problem is rather a restrictive approach.   Lymphoproliferative disorders related to other histotypes (tissue types from the growth of a tumour) [7]. EBV-associated T-cell and NK-cell lymphoproliferative disorders include [7]: Peripheral T-cell lymphoma; Chronic active EBV infection of T-cell and NK-cell types (with cutaneous and systemic forms) Angioimmunoblastic T-cell lymphoma.

Psoriasis is an immune-mediated inflammatory dermatosis characterized by epidermal hyperplasia and excessive infiltration of inflammatory cells. Withanolides, extracted from Datura metel L.; are the main effective components for the treatment of psoriasis. However, the precise mechanisms of action of withanolides for the treatment of psoriasis remain unclear. Other bullous disorder: Diseases of the Dermis and Subcutaneous Tissue Disease of Pigmentation; Atrophodermas Diseases of collagen and elastic tissue Disorders of vascular tissue Disorders of mast cells, including urticaria Diseases of the hair and scalp Acne and related disorders: Hyperpigmentation, metabolic, post-inflammatory, or idiopathic. the proliferative stimulus due to the biopsy scar caused clonal proliferation of T cells, then may lead to the development of lymphoproliferative disorder.3 The pathophysiology of the appearance of lymphoprolif-erative disorders in scars or sites of skin trauma is unknown. 4 It has been postulated that proliferative stimuli secondary to. Normal-appearing epidermis from patients with psoriasis appears to have a higher proliferation rate. The mean LI is % +/- , approximately 50% greater than in normal epidermis. Absolute cell kinetic values for this tissue, however, cannot yet be calculated for lack of other information on ts and GF.

Immunohistochemical evaluation of psoriasis and other proliferative disorders of the skin. by John Patrick Harford Download PDF EPUB FB2

Background/purpose: The aims of this study were to investigate the clinical and immunohistochemical events of psoriatic plaques during photodynamic therapy (PDT) using topical application of 5‐aminolaevulinic acid (ALA).

Methods: Twelve psoriatic patients were recruited for this study. Four of them dropped out because of pain during treatment.

The effect of PDT was evaluated Cited by: Introduction. The pathogenesis of psoriasis vulgaris, a chronic, inflammatory skin disorder, is far from being elucidated. It is considered a multifactorial disease, involving the interaction of immune mediated processes, genetic background and environmental factors (1,2).In response, a series of changes will appear: dilated capillaries in the dermis due to enhanced angiogenesis Author: Iulia-Ioana Morar, Flaviu-Alexandru Tabăran, Teodora Mocan, Elena-Mihaela Jianu, Meda-Sandra Orăsan.

Immunohistochemical evaluation of Ki and caspase 3 expressions in OSE cells. The expression of tested proteins has shown statistically significant differences among analyzed groups (Figs.

3 and 4).The existence of positive expression of the nuclear antigen Ki in OSE cells was observed to be definitely rare in the study group, as compared to the control group (p Cited by: 9.

Since psoriasis is a hyperproliferative skin disorder, it seemed reasonable that the anti-proliferative effects of 1,25(OH) 2 D 3 could be used for the treatment of this disease.

Based on various research findings, it can be speculated that innovative vitamin D analogs will introduce novel alternatives for the treatment of various skin by: 6.

Request PDF | Psoriasis and Other Skin Diseases | Psoriasis is a chronic dermatosis of unknown etiology characterized by skin inflammation, hyper proliferation and altered differentiation of. Vitamin D is photochemically synthesized by ultraviolet-B (UV-B) action in the skin, [also see Chapter 4 (vol.

1 of this book)]. It is known that the skin itself is a target tissue for the seco-steroid hormone 1,25(OH) 2 D 3, the biologically active vitamin D metabolite. 1,25(OH) 2 D 3 exerts genomic and nongenomic effects.

Nongenomic effects of 1,25(OH) 2 D 3 and analogs are, in part. Psoriasis is a common chronic inflammatory and hyperproliferative skin disorder characterized by patches of thick, red skin covered with silvery scales [1]. The disorder affects approximately %. Psoriasis represents a chronic inflammatory skin disease with multisystemic involvement.

The development of this autoimmune disorder depends on a complex interplay of genetic and environmental factors. Besides presenting the conditions associated with psoriasis, the chapter outlines the role of hormones (sex hormones, prolactin, and thyroid hormones) in psoriasis pathogenesis and evolution.

Immunohistochemical analysis was performed in human skin sections using a standard procedure, and the histologic evaluation was carried out in the Department of Pathology, Tartu University Hospital.

In brief, formalin-fixed paraffin-embedded sections from normal and psoriasis skin specimens were cut at 3 μm onto adhesive-coated slides. PROSE Award Winner - Multivolume Reference/Science The world's number 1 dermatology information resource.

Universally respected, Rook's Textbook of Dermatology is the most comprehensive, definitive and best-illustrated reference work for dermatologists of all levels worldwide and has been at the forefront of international dermatology publishing since first appearing in Psoriasis vulgaris, a chronic inflammatory skin disorder, is the result of immune mediated processes, genetic background and environmental factors.

Prolactin and the vascular endothelial growth factor seem to play a key role in psoriasis pathogenesis regarding hyperproliferation of epidermal keratinocytes and dermal vascular ectasia. Psoriasis is a common chronic skin disorder affecting 2% of the general population.

Present evidence strongly suggests that it is an immunologically mediated disease; the evidence includes the results of disease association studies linking psoriasis to certain MHC antigens and immunohistochemical studies revealing early influx into lesions of activated T lymphocytes.

Psoriasis is a persistent chronic relapsing inflammatory skin disorder. It is considered as a hyper proliferative disease with immune-mediated basis and genetic predisposition.

About 2%-3% of the population is affected by psoriasis. The lesions usually consist of red plaques with scales, sharp demarcation and indurations. Background and Objective: Psoriasis is a chronic inflammatory skin disease characterized by hyper-proliferation, abnormal differentiation, and inflammatory infiltration in epidermis and dermis.

We planned this study to analyze probable associations between Osteopontin (OPN), Ki, CD34, and histopathological features in psoriasis. Psoriasis (PS) is a systemic, chronic, relapsing and hyperproliferative immune-mediated skin disorder.

It is characterized by inflammatory skin and joint manifestations.[ 2 ] PS results from interaction between genetic predisposition and large spectrum of environmental factors that triggers the development of skin.

Psoriasis is a chronic inflammatory dermatologic disease, affecting 2–3% of the adult population and –% of children [1,2].Patients with psoriasis vulgaris present with scaly, erythematous plaques on the skin, and the skin lesions are usually accompanied by an infiltration of immune cells and accelerated proliferation of keratinocytes and endothelial cells [].

Psoriasis is a chronic skin disorder that manifests as epidermal keratinocyte hyperplasia. Objectives. We examined the effect of oxymatrine treatment on cell proliferation and apoptosis in skin lesions of psoriasis.

Patients and methods. Patients with severe plaque psoriasis. In 2, patients suffering from psoriasis, evaluation of the age of onset revealed two peaks, one occurring at the age of 16 years (female) or 22 years (males) and a second peak at the age of The review also broke out risk by different forms of cancer and found that severe psoriasis was linked with a more than 11 times higher risk for squamous cell carcinoma (a common form of skin.

Setting the Stage: Psoriasis as an Immune-Mediated Disorder. If I was to name diseases that in recent years have increased our understanding of both adaptive and innate immune mechanisms on the one hand and have contributed decisively to the development of modern biological therapies on the other, then psoriasis would certainly occupy one of the top ranks.

Psoriasis is a common inflammatory disorder of the skin, characterized by excessive proliferation and abnormal differentiation of keratinocytes [].Psoriasis is classified into five morphological subtypes, among which plaque and guttate psoriasis are the most common clinical phenotypes [].Some studies have shown that plaque and guttate psoriasis are associated with Th1 cytokines and Th in psoriatic skin by immunohistochemical analysis and to evaluate the correlation with disease severity.

Two skin biop-sies, psoriatic lesion and perilesional skin, obtained by punch biopsy from 19 nontreated psoriasis patients were examined in hematoxylin and eosin staining and immunohistochem-istry α(IHC) for TNF- VEGFR2 and PRLR.

The indirect. In contrast, CALML3 may be aberrantly expressed and/or downregulated in hyperproliferative disorders such as psoriasis and ichthyosis, as well as in pre-cancerous lesions (such as AK) and in squamous cell and basal cell non-melanoma skin cancers. We tested this by immunohistochemistry on biopsies representing different skin disorders.

Introduction. Psoriasis is a chronic, disabling skin disorder, affecting approximately 2% of the Western population. Psoriasis is characterized by epidermal hyperplasia due to high keratocytes proliferation rate, abnormal keratocytes differentiation, infiltration of the skin by activated lymphocytes and neutrophils.

The prevalence of skin disease in India is 10 to 12 percent of the total population with Eczema and Psoriasis being the major contributors. Due to pollution, ultraviolet light, and global warming, photosensitive skin disorders like pigment darkening, sunburn, skin cancers, and infectious diseases are increasing at a faster pace.

Psoriasis is a chronic skin condition that is often associated with systemic manifestations, especially arthritis. An estimated 2 percent of. Platelet-rich plasma (PRP) therapy has been considered as a promising treatment for androgenetic alopecia (AGA).

The aim of the study was comparative evaluation of the clinical efficacy of PRP-therapy, minoxidil, and their combination in the treatment of men with AGA and to evaluate the effects of PRP on the proliferation of hair follicle (HF) cells in skin biopsy.

Materials and Methods: The. Since psoriasis is a hyperproliferative skin disorder, it seemed reasonable that the anti-proliferative effects of 1,25(OH) 2 D 3 could be used for the treatment of this disease.

However, before launching clinical trials inMacLaughlin and associates reported the observation that psoriatic fibroblasts are partially resistant to the anti. Psoriasis is a hyperproliferative disorder of keratinocytes.9 The lesions of psoriasis are distinctive. They begin as red, scaling papules that coalesce to form round-to-oval plaques, easily distinguishable from the surrounding normal skin The primary psoriatic lesion is an erythematous papule topped by a loosely adherent scale.

Psoriasis is an incurable skin disorder characterized by the presence of inflammatory plaques on the skin. Although there are multiple therapeutic options to treat this disease, patients worldwide.

Psoriasis is an immune-mediated, genetic disease manifesting in the skin or joints or both. A diverse team of clinicians with a range of expertise is often needed to treat the disease.

Psoriasis provides many challenges including high prevalence, chronicity, disfiguration, disability, and associated comorbidity. Understanding the role of immune function in psoriasis and the interplay between.Psoriasis is an erythematous, scaly chronic inflammatory dermatosis and occurs due to altered epidermal differentiation and hyperproliferation due to faulty signals that speed up the growth cycle of skin cells.

Psoriasis reduces quality of life, and psoriatic patients generally have higher risk for metabolic disease. Psoriasis is associated with many burdening comorbidities, which often share.Psoriasis is a common chronic inflammatory disease of the skin that is increasingly being recognized as a systemic inflammatory disorder.

Psoriatic arthritis is a well-known comorbidity of psoriasis.